748003 VU Applications of Chemical Ionisation Mass Spectrometry to Trace Gas Analysis
Wintersemester 2024/2025 | Stand: 17.10.2024 | LV auf Merkliste setzen- To introduce state-of-the-art soft chemical ionisation mass spectrometric (SCIMS) techniques, namely; Proton Transfer Reaction – Time-of-Flight – Mass Spectrometry (PTR-ToF-MS), Selected Ion Flow Tube – Mass Spectrometry (SIFT-MS) and Ion Mobility Spectrometry (IMS)
- Compare SCIMS to more standard mass spectrometric analytical techniques including the “gold-standard” of analytical chemistry; namely Gas Chromatography – Mass Spectrometry (GC-MS) – advantages and disadvantages
- To provide a framework for gaining a knowledge and an understanding on the use of reagent ions as sensitive and selective analytical probes for use in many areas of analytical chemistry – interdisciplinary and intersectoral perspectives
- Comparison of SCIMS to more standard mass spectrometric analytical techniques including the “gold-standard” of analytical chemistry; namely Gas Chromatography – Mass Spectrometry (GC-MS) – advantages and disadvantages
- To provide a major practical hands-on research project within the Institute for Breath Research using state-of-the-art PTR-MS instruments – students will become proficient in using PTR-MS and in the analysis of the mass spectrometric data obtained:
- putting the context of the lectures into real-world applications
- gaining experience of working in a research environment (team and independent work)
- gaining skills in the use of using state-of-the-art soft chemical ionisation mass spectrometric and analysis techniques (An Ionicon Analytik GmbH PTR-TOF 6000 X2)
- developing experimental, critical thinking and analytical skills
- gaining experience in writing and presenting scientific reports
1. Lecture Material
Analytical performance of a SCIMS instrument-Ions as Sensitive Analytical Probes to the Environment
Accuracy and precision, Sensitivity, Limits of detection, Mass accuracy, Selectivity, Use of ion-molecule reaction rate coefficients to determine compound concentration
Principles of Proton Transfer Reaction Mass Spectrometry
Non-dissociative and dissociative proton transfer, Operational principles, Soft ionisation versus electron impact ionisation, Determining concentrations, Selectivity
Proton Transfer Reaction Mass Spectrometry and the Reduced Electric Field
Residence times, Mean drift velocities, Collisional energies (Wannier equation) and effective temperatures, Sensitivity, Limits of detection
Proton Transfer Reaction Mass Spectrometry: Improving Selectivity and Response Time
Selectivity of PTR-MS – methods employed to improve selectivity, Response Time of Measurements with PTR-MS – changing reagent ions, Latest PTR-MS developments
Selected Ion Flow Tube Mass Spectrometry (SIFT-MS)
Operational principles, Determining absolute rate coefficients, Use in trace gas analysis – commercialisation of SIFT-MS, Advantages and disadvantages of SIFT-MS
Ion Mobility Spectrometry (IMS)–Principles and Methods
Operational principles, Mobility coefficient, Mean drift velocity, Reagent ion peak, IMS spectra, Ionisation sources, IMS-MS, Applications of IMS, Advantages and disadvantages of IMS
Ion Mobility Spectrometry (IMS)-Positive Ion Mode
Reagent ion formation, Applications (CWAs and Drugs)
Ion Mobility Spectrometry (IMS)-Negative Ion Mode
Reagent ion formation, Applications (Explosives), Electron attachment investigations – IMS and swarm measurements
IMS and Improved Selectivity
Dopants, GC-IMS, Collisional Induced Dissociation, High Kinetic Energy Ion Mobility Spectrometry
Soft Chemical Ionisation Mass Spectrometry and Atmospheric Chemistry
The biosphere, Plant studies and influences on the atmosphere, Chamber studies, Emission monitoring, Biomass burning, Airborne and shipborne measurements
Soft Chemical Ionisation Mass Spectrometry and Food Science
Mass spectral fingerprinting, Flavour release and perception, Food classification, Food freshness and ripening
Soft Chemical Ionisation Mass Spectrometry and Homeland Security
Thermal desorption PTR-MS, Explosive detection with PTR-MS
Soft Chemical Ionisation Mass Spectrometry and Health Sciences
Breath analysis, Urine analysis, Skin emanations, Endogenous verses exogenous volatile sources, PTR-MS and breath research, Volatiles and breath tests in clinical practice
Electronic Sensors–Future Directions
Chemical recognition by chemical gas sensors
2. Research Project (5 days)–Applications of PTR-MS to Breath Analysis
Extensive training on the operation of the state-of-the-art PTR-ToF-MS (an Ionicon Analytik GmbH PTR-TOF 6000 X2) and analyses of data.
Measure the breath concentrations (VMRs) of key volatiles in breath, Calculate the breath concentrations and compare to measured values, Investigate temporal changes of breath volatile concentrations (pharmacokinetics)
Lectures and Experimental Work (taught in English)
To provide a series of tutorial style lectures drawing together and developing concepts from various disciplines (e.g. chemistry, physics, and biosciences) and different subjects (e.g. quantum mechanics, plasma physics, statistical mechanics, and electromagnetism) to describe the applications of ion-molecule processes for use in gas-phase analytical chemistry.
G.A. Eiceman, Z. Karpas, H. H. Hill „Ion Mobility Spectrometry” - ISBN: 9781138199484
(CRC Press 2016)
A. M. Ellis and C. A. Mayhew “Proton Transfer Reaction Mass Spectrometry: Principles and Applications - ISBN: 978-1-4051-7668-2 (Wiley 2014)” 350 pages (provided)
VO Fundamentals of Chemical Ionisation Mass Spectrometry
Gruppe | Anmeldefrist | |
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748003-0 | 01.09.2024 00:00 - 31.01.2025 23:59 | Zur LV anmelden |